6RUT

Mycoplasma Genitalium Heterodimer Nap Complex (P140-P110 globular)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-05-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97897
Spacegroup name	P 1 21 1
Unit cell lengths	151.000, 157.280, 192.370
Unit cell angles	90.00, 93.81, 90.00

Refinement procedure

Resolution	121.660 - 2.650
R-factor	0.189
Rwork	0.187
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6r3t
RMSD bond length	0.010
RMSD bond angle	1.250
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	157.280	2.700
High resolution limit [Å]	2.650	2.650
Number of reflections	189359	784
<I/σ(I)>	6.6
Completeness [%]	73.3
Redundancy	6.8
CC(1/2)	0.873	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	23% PEG MME 4% PGA 0.1M Sodium cacodylate pH 6.5