6RUP

Human mitochondrial single-stranded DNA binding protein, SSBP1, at 2.1 A resolution - elucidated sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-28
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.979500
Spacegroup name	P 41 21 2
Unit cell lengths	51.040, 51.040, 182.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.580 - 2.100
R-factor	0.202
Rwork	0.200
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ull
RMSD bond length	0.008
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.580	2.230
High resolution limit [Å]	2.100	2.100
Rmeas	0.096	0.245
Number of reflections	14943	2342
<I/σ(I)>	16.28
Completeness [%]	99.5	97.5
Redundancy	11	11.2
CC(1/2)	0.999	0.542

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	293	11 % PEG1500, 0.1 M cacodylate pH 6.5, 0.2 M magnesium chloride. cryoprotectant solution included