6RUM
Crystal structure of GFP-LAMA-G97 - a GFP enhancer nanobody with cpDHFR insertion and TMP and NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.890, 56.580, 53.290 |
| Unit cell angles | 90.00, 94.91, 90.00 |
Refinement procedure
| Resolution | 47.715 - 1.600 |
| R-factor | 0.1852 |
| Rwork | 0.183 |
| R-free | 0.22750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uii 5h8d |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.957 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14rc1_3177: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.054 | 0.490 |
| Number of reflections | 37239 | 6123 |
| <I/σ(I)> | 13.23 | 2.43 |
| Completeness [%] | 99.1 | 98.6 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M MES pH 6.0, 20% (w/v) PEG 6000, 1.0 M lithium chloride |
