6RUM
Crystal structure of GFP-LAMA-G97 - a GFP enhancer nanobody with cpDHFR insertion and TMP and NADPH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.99999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.890, 56.580, 53.290 |
Unit cell angles | 90.00, 94.91, 90.00 |
Refinement procedure
Resolution | 47.715 - 1.600 |
R-factor | 0.1852 |
Rwork | 0.183 |
R-free | 0.22750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uii 5h8d |
RMSD bond length | 0.006 |
RMSD bond angle | 0.957 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14rc1_3177: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.054 | 0.490 |
Number of reflections | 37239 | 6123 |
<I/σ(I)> | 13.23 | 2.43 |
Completeness [%] | 99.1 | 98.6 |
Redundancy | 3.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1 M MES pH 6.0, 20% (w/v) PEG 6000, 1.0 M lithium chloride |