6RUL
Crystal structure of GFP-LAMA-F98 - a GFP enhancer nanobody with cpDHFR insertion and TMP and NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97797 |
| Spacegroup name | P 62 |
| Unit cell lengths | 86.610, 86.610, 73.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.305 - 2.200 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.21950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uii 5h8d |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.068 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14rc1_3177: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.058 | 0.453 |
| Number of reflections | 15911 | 1970 |
| <I/σ(I)> | 22.62 | 5.32 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10.1 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M MES pH 6.0, 30% (v/v) PEG 600, 5% (w/v) PEG 1000, 10% (v/v) glycerol |
