6RTH
Crystal structure of the ligand-free glycosyltransferase domain from the YGT toxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.178, 161.737, 162.971 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.400 |
| R-factor | 0.228 |
| Rwork | 0.227 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rtg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.670 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.473 | 1.508 |
| Number of reflections | 17726 | 3590 |
| <I/σ(I)> | 7 | 2.2 |
| Completeness [%] | 99.7 | |
| Redundancy | 13 | 12.2 |
| CC(1/2) | 0.969 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 200 mM sodium formate, 20% PEG 3350 |
