6RT9

Orthorhombic lysozyme grown with 300g/L sucrose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION GEMINI
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-19
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54056
Spacegroup name	P 21 21 21
Unit cell lengths	30.065, 56.091, 72.455
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.785 - 1.550
R-factor	0.1863
Rwork	0.184
R-free	0.22780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2w1x
RMSD bond length	0.005
RMSD bond angle	0.887
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.785	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.071	0.872
Rmeas	0.085
Rpim	0.044	0.754
Number of reflections	18289	859
<I/σ(I)>	11.7	0.8
Completeness [%]	99.1	95.5
Redundancy	3.3	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7	310	0.2M Sodium Acetate pH 4.7, 3% (w/v) NaCl, 300 g/L sucrose