6RT4

The YTH domain of YTHDC1 protein in complex with m6ACU oligonucleotide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000002
Spacegroup name	P 1 21 1
Unit cell lengths	40.105, 103.503, 41.955
Unit cell angles	90.00, 104.69, 90.00

Refinement procedure

Resolution	38.794 - 1.490
R-factor	0.2053
Rwork	0.204
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3h
RMSD bond length	0.005
RMSD bond angle	0.785
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.870	1.580
High resolution limit [Å]	1.490	1.490
Rmeas	0.037	0.597
Number of reflections	53214	8210
<I/σ(I)>	17.15	1.9
Completeness [%]	95.1	93.9
Redundancy	3.47	3.26
CC(1/2)	0.999	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Bis-Tris at pH 6.5, 0.2 M ammonium sulfate and 30% PEG 3350