6RSY
The complex between TCR a7b2 and human Class I MHC HLA-A0201-WT1 with the bound RMFPNAPYL peptide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2018-09-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9159 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.610, 114.750, 185.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 97.760 - 2.950 |
| R-factor | 0.25275 |
| Rwork | 0.251 |
| R-free | 0.29115 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.254 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 114.750 | 3.000 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.346 | 2.016 |
| Number of reflections | 43245 | 43245 |
| <I/σ(I)> | 8.8 | 1.2 |
| Completeness [%] | 100.0 | 99.95 |
| Redundancy | 14.1 | 14 |
| CC(1/2) | 0.524 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 7.5% PEG 8 K , 0.75 M NaCl , 50mM HEPES , pH 7.0 |
