6RSX

Regulatory Subunit of cAMP-dependant Protein Kinase A from Euglena gracilis at 1.6 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000
Spacegroup name	P 61
Unit cell lengths	80.586, 80.586, 110.755
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	69.790 - 1.600
R-factor	0.193489922461
Rwork	0.192
R-free	0.21630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rgs
RMSD bond length	0.011
RMSD bond angle	1.084
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.790	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.073	0.081
Rmeas		0.085
Number of reflections	52962	7528
<I/σ(I)>	14.33
Completeness [%]	99.6
Redundancy	10.1
CC(1/2)	0.999	0.483

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	1) 50mM MES pH 6.0 , 4% MPD , 80mM Ammonium sulfate , 18% PEG 8000 2) 50mM MES pH 6.0, 20% PEG 3350 3) 50mM MES pH 6.0 , 4% MPD , 0.2M Ammonium sulfate , 20% PEG 3350