6RRR
Crystal structure of the tyrosinase PvdP from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-22 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.162, 112.616, 100.265 |
Unit cell angles | 90.00, 92.71, 90.00 |
Refinement procedure
Resolution | 49.130 - 2.110 |
R-factor | 0.1717 |
Rwork | 0.169 |
R-free | 0.22000 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.544 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.170 |
High resolution limit [Å] | 2.110 | 2.110 |
Rmerge | 0.080 | 0.657 |
Rmeas | 0.091 | 0.742 |
Rpim | 0.042 | 0.339 |
Number of reflections | 55955 | 4391 |
<I/σ(I)> | 12.2 | |
Completeness [%] | 99.4 | 95.7 |
Redundancy | 4.5 | 4.5 |
CC(1/2) | 0.998 | 0.841 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 20% PEG 3350, 0.1M Bis-Tris Propane |