6RR0
Crystal structure of the Sir4 H-BRCT domain in complex with Ubp10 pT123 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.040, 75.280, 95.910 |
| Unit cell angles | 90.00, 97.24, 90.00 |
Refinement procedure
| Resolution | 47.570 - 2.180 |
| R-factor | 0.1975 |
| Rwork | 0.196 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Number of reflections | 51736 | 3797 |
| <I/σ(I)> | 9.51 | 1.02 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.8 | 6.8 |
| CC(1/2) | 0.998 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 293 | 27.8 % PEG 4000 0.1 M sodium acetate pH 4.9 0.2 M ammonium acetate 3% w/v trimethylamine N-oxide dehydrate |
