6RPX

Cytokine receptor-like factor 3 C-terminus residues 174-442: native

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	88.030, 40.380, 76.530
Unit cell angles	90.00, 99.94, 90.00

Refinement procedure

Resolution	37.691 - 1.610
R-factor	0.1785
Rwork	0.177
R-free	0.20120
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.066
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.691	1.650
High resolution limit [Å]	1.610	1.610
Rmerge	0.045	0.764
Rmeas	0.063	1.053
Number of reflections	33326	2356
<I/σ(I)>	13.3	1.4
Completeness [%]	96.7	94.9
Redundancy	3.4	3.6
CC(1/2)	0.999	0.504

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	25 % PEG 3,350, 0.2 M ammonium acetate, 0.1 M Bis-Tris. Cryoprotected in perfluoropolyether oil.