6RPX
Cytokine receptor-like factor 3 C-terminus residues 174-442: native
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.030, 40.380, 76.530 |
| Unit cell angles | 90.00, 99.94, 90.00 |
Refinement procedure
| Resolution | 37.691 - 1.610 |
| R-factor | 0.1785 |
| Rwork | 0.177 |
| R-free | 0.20120 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.066 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.691 | 1.650 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.045 | 0.764 |
| Rmeas | 0.063 | 1.053 |
| Number of reflections | 33326 | 2356 |
| <I/σ(I)> | 13.3 | 1.4 |
| Completeness [%] | 96.7 | 94.9 |
| Redundancy | 3.4 | 3.6 |
| CC(1/2) | 0.999 | 0.504 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 25 % PEG 3,350, 0.2 M ammonium acetate, 0.1 M Bis-Tris. Cryoprotected in perfluoropolyether oil. |
