6RP0
The crystal structure of a complex between the LlFpg protein, a THF-DNA and an inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 92.180, 92.180, 139.726 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.570 - 2.250 |
R-factor | 0.1654 |
Rwork | 0.164 |
R-free | 0.20190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pm5 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.181 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.660 | 2.370 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.060 | |
Number of reflections | 29044 | 4126 |
<I/σ(I)> | 14.3 | 1.9 |
Completeness [%] | 99.4 | 99.2 |
Redundancy | 3.7 | |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | HEPES, CITRATE |