6ROZ

Structure of the N-SH2 domain of the human tyrosine-protein phosphatase non-receptor type 11 in complex with the phosphorylated immune receptor tyrosine-based switch motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-22
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00002
Spacegroup name	P 43
Unit cell lengths	30.035, 30.035, 213.113
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.280 - 2.890
R-factor	0.24372
Rwork	0.239
R-free	0.28462
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tkz
RMSD bond length	0.008
RMSD bond angle	1.252
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0171)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.280	3.070
High resolution limit [Å]	2.890	2.890
Rmerge	0.182	1.316
Rpim	0.074	0.552
Number of reflections	4283	713
<I/σ(I)>	10.4	2
Completeness [%]	100.0	100
Redundancy	13.8	13.2
CC(1/2)	0.998	0.764

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.1	293	31.7% (w/v) PEG 3350, 0.1 M HEPES, 0.233 M MgSO4