6ROA
Crystal structure of V57G mutant of human cystatin C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 61 |
| Unit cell lengths | 75.828, 75.828, 98.176 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.650 |
| R-factor | 0.17819 |
| Rwork | 0.174 |
| R-free | 0.25238 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nx0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.715 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.127 | 0.565 |
| Number of reflections | 9095 | 9095 |
| <I/σ(I)> | 13.7 | 2.05 |
| Completeness [%] | 96.8 | 96.2 |
| Redundancy | 4.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M Na acetate, 0.1M Na cacodylate pH 6.5, 30% PEG 8000. |
