6RNQ

Crystal structure of the dimerization domain of Gemin5 at 1.95 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	64.219, 54.972, 103.640
Unit cell angles	90.00, 104.30, 90.00

Refinement procedure

Resolution	100.400 - 1.950
R-factor	0.2118
Rwork	0.211
R-free	0.23540
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	0.874
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol (1.13_2998)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.400	2.020
High resolution limit [Å]	1.950	1.950
Number of reflections	51558	5087
<I/σ(I)>	9.96
Completeness [%]	99.2
Redundancy	3.4
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	200 mM NaK Tartrate, 25% PEG 3350 and 100 mM BisTris Methane pH 8.5