6RNN

P46, an immunodominant surface protein from Mycoplasma hyopneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	84.209, 88.380, 123.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.000 - 1.950
Rwork	0.207
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a3r
RMSD bond length	0.007
RMSD bond angle	0.955
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((dev_2880: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.000	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	94930	5588
<I/σ(I)>	5.92
Completeness [%]	94.0
Redundancy	18.4
CC(1/2)	0.980	0.270

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	0.1 M Sodium Citrate pH 4.0 21% PEG6000