6RNM
Crystal structure of a complex between the LlFpg protein, a THF-DNA and an inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-11 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.96770 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.957, 91.957, 142.182 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.130 - 1.760 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pm5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.130 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.056 | 0.957 |
| Number of reflections | 61222 | 3017 |
| <I/σ(I)> | 18.3 | |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 7.2 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | HEPES, SODIUM CITRATE |
