6RNB

Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Lysozyme with GlcNAc3 50ms diffusion time

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	293
Detector technology	PIXEL
Collection date	2019-04-27
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	78.700, 78.700, 37.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.649 - 1.699
R-factor	0.1701
Rwork	0.169
R-free	0.18620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5njr
RMSD bond length	0.009
RMSD bond angle	1.157
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.649	1.830
High resolution limit [Å]	1.699	1.699
Number of reflections	13641	1334
<I/σ(I)>	7.6
Completeness [%]	100.0
Redundancy	502.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	20 mM NaAcetate pH 4.7, 50 mM NaCl