6RN5
PptA from Streptomyces chartreusis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.905002 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 49.303, 132.233, 116.398 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.196 - 2.037 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.532 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0218 2018/15/02) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.196 | 2.160 |
| High resolution limit [Å] | 2.037 | 2.037 |
| Rmerge | 0.106 | 0.916 |
| Rmeas | 0.111 | 0.971 |
| Number of reflections | 24547 | 3733 |
| <I/σ(I)> | 19.2 | 2.26 |
| Completeness [%] | 98.9 | 94.5 |
| Redundancy | 12.3 | 8.9 |
| CC(1/2) | 0.999 | 0.711 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 100 mM MES/HCl pH 6.5, 1.6 M MgSO4 |
