6RLZ
Crystal Structure of 14-3-3zeta in complex with a macrocyclic 8-mer peptide derived from ExoS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97870 |
| Spacegroup name | P 64 |
| Unit cell lengths | 98.800, 98.800, 94.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.810 - 3.700 |
| R-factor | 0.22336 |
| Rwork | 0.216 |
| R-free | 0.29220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.841 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 10.000 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Number of reflections | 5633 | 186022 |
| <I/σ(I)> | 16.02 | |
| Completeness [%] | 99.1 | |
| Redundancy | 33 | |
| CC(1/2) | 0.992 | 0.921 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M Magnesium formate 20% PEG 3350 pH 7.5 |
