6RLR
Crystal structure of CD9 large extracellular loop
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.986, 39.998, 63.643 |
| Unit cell angles | 80.39, 76.29, 68.15 |
Refinement procedure
| Resolution | 29.017 - 2.000 |
| R-factor | 0.241 |
| Rwork | 0.239 |
| R-free | 0.27940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rlo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.393 |
| Data reduction software | EVAL15 |
| Data scaling software | EVAL15 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.017 | 2.072 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.121 | 1.364 |
| Rpim | 0.065 | 0.732 |
| Number of reflections | 22863 | 2180 |
| <I/σ(I)> | 4.82 | 0.82 |
| Completeness [%] | 95.6 | 93.003 |
| Redundancy | 3.407 | 3.355 |
| CC(1/2) | 0.991 | 0.322 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293.15 | pentaerythritol propoxylate, potassium chloride, sodium citrate |
