6RLQ

CRYSTAL STRUCTURE OF THE HUMAN PRMT5:MEP50 COMPLEX with JNJ45031882

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	I 2 2 2
Unit cell lengths	101.132, 137.854, 178.617
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.300 - 2.530
R-factor	0.2334
Rwork	0.230
R-free	0.29940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.897
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.300	2.600
High resolution limit [Å]	2.530	2.530
Number of reflections	275414	3023
<I/σ(I)>	11.2
Completeness [%]	98.9
Redundancy	6.6
CC(1/2)	0.865	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	278	30-50% PEG3350, 150mM Ammonium Sulphate