6RLG
Crystal structure of LdtMt2 from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-30 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97952 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.950, 93.920, 75.570 |
| Unit cell angles | 90.00, 93.03, 90.00 |
Refinement procedure
| Resolution | 33.787 - 1.510 |
| R-factor | 0.181402239935 |
| Rwork | 0.180 |
| R-free | 0.20383 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5duj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.993 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.790 | 1.540 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.075 | |
| Rmeas | 0.081 | |
| Rpim | 0.032 | 0.685 |
| Number of reflections | 132783 | 6593 |
| <I/σ(I)> | 12.03 | 1.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.5 | 6.7 |
| CC(1/2) | 0.990 | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 277 | 0.2 M Ammonium nitrate pH 6.3, 20% PEG 3350 |
