6RL1
The crystal structure of AbnE, an arabino-oligosaccharide binding protein, in complex with arabinotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.088, 120.230, 39.474 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.614 - 1.700 |
| R-factor | 0.1669 |
| Rwork | 0.166 |
| R-free | 0.19960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rkh |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.040 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.064 | 0.427 |
| Number of reflections | 54441 | 7085 |
| <I/σ(I)> | 29.5 | 5.5 |
| Completeness [%] | 97.7 | 86 |
| Redundancy | 13.7 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 22% PEG 4K, 0.1M Tris/HCl buffer, pH 9 |
