6RKF
Structure of human DASPO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-10-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 114.397, 159.715, 288.575 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 93.002 - 3.219 |
| R-factor | 0.2328 |
| Rwork | 0.231 |
| R-free | 0.27080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dao |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.727 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.15_3448: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 144.290 | 3.660 |
| High resolution limit [Å] | 3.219 | 3.219 |
| Rpim | 0.133 | 0.518 |
| Number of reflections | 23134 | 114 |
| <I/σ(I)> | 5.9 | 1.6 |
| Completeness [%] | 53.5 | 8.7 |
| Redundancy | 5.8 | 5.5 |
| CC(1/2) | 0.986 | 0.535 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 291 | 20 mM Tris-HCl pH 8.0, 10 % glycerol, 50 mM NaCl |
