6RK7
Inter-dimeric interface controls function and stability of S-methionine adenosyltransferase from U. urealiticum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-27 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.976 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 113.999, 106.139, 234.377 |
Unit cell angles | 90.00, 96.86, 90.00 |
Refinement procedure
Resolution | 48.660 - 1.800 |
R-factor | 0.17017 |
Rwork | 0.169 |
R-free | 0.19448 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rjs |
RMSD bond length | 0.023 |
RMSD bond angle | 2.154 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.660 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.033 | 0.566 |
Number of reflections | 255563 | 12581 |
<I/σ(I)> | 9.4 | |
Completeness [%] | 99.8 | |
Redundancy | 4.7 | |
CC(1/2) | 0.996 | 0.503 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Hepes pH 7.0 and 30% Jeffamine ED-2001 |