6RK6

Characterization of an intertidal zone metagenome oligoribonuclease and the role of the intermolecular disulfide bond for homodimer formation and nuclease activity.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.91841
Spacegroup name	P 31 2 1
Unit cell lengths	108.322, 108.322, 101.334
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.580 - 3.150
R-factor	0.2467
Rwork	0.245
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gbz
RMSD bond length	0.003
RMSD bond angle	0.678
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.580	3.370
High resolution limit [Å]	3.150	3.150
Rpim	0.076	0.551
Number of reflections	12264	2200
<I/σ(I)>	8.2	1.6
Completeness [%]	99.9	100
Redundancy	5.2	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.1 M Hepes, pH 7.0, 5% (v/v) PEG 8000.