6RK1

Crystal structure of TSP1 domain from CCN3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.930
Spacegroup name	P 31 2 1
Unit cell lengths	52.860, 52.860, 102.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.130 - 1.630
R-factor	0.1626
Rwork	0.162
R-free	0.18440
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.751
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.130	1.660
High resolution limit [Å]	1.630	1.630
Rmerge	0.082
Number of reflections	2082	220
<I/σ(I)>	13
Completeness [%]	100.0
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	3.0 M NaCl, 0.1 M Tris pH 8.0