6RHE
CpOGA D298N in complex with hOGA-derived S-GlcNAc peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 61 |
| Unit cell lengths | 117.651, 117.651, 147.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.170 - 3.100 |
| R-factor | 0.19133 |
| Rwork | 0.189 |
| R-free | 0.23339 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ydq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.666 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.170 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rpim | 0.090 | 0.720 |
| Number of reflections | 19800 | 3629 |
| <I/σ(I)> | 8 | 1.5 |
| Completeness [%] | 94.1 | 95.5 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1:1 drops of proytein mixed with crystallisation buffer: 0.175 M CdSO4 and 0.6 M NaAc |
