6RGV

Truncated FljB phase 2 flagellin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-03
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	C 1 2 1
Unit cell lengths	97.468, 38.280, 124.690
Unit cell angles	90.00, 103.54, 90.00

Refinement procedure

Resolution	50.000 - 2.000
Rwork	0.213
R-free	0.25850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1io1
RMSD bond length	0.008
RMSD bond angle	1.325
Data reduction software	XDS (December 6, 2010)
Data scaling software	XSCALE (December 6, 2010)
Phasing software	PHASER (2.5.2)
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.050
High resolution limit [Å]	2.000	8.930	2.000
Rmerge	0.096	0.036	0.673
Rmeas	0.106	0.040	0.752
Total number of observations	169968
Number of reflections	30567	388	1927
<I/σ(I)>	11.25	25.66	2.26
Completeness [%]	98.7	99.5	86
Redundancy	5.561	5.418	4.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	0.1 M Tris (pH 8.5), 20% (w/v) PEG4000, 24% (v/v) isopropanol