6RFT

Crystal structure of Eis2 from Mycobacterium abscessus bound to Acetyl-CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-31
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.973
Spacegroup name	P 1 21 1
Unit cell lengths	111.650, 76.990, 152.560
Unit cell angles	90.00, 90.81, 90.00

Refinement procedure

Resolution	48.939 - 2.300
R-factor	0.2297
Rwork	0.229
R-free	0.26660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.506
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX ((1.15rc3_3435: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.939	2.400
High resolution limit [Å]	2.300	2.300
Rmeas	0.224
Number of reflections	115359	13735
<I/σ(I)>	8.35
Completeness [%]	100.0	100
Redundancy	6.81

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.1 M BIS-TRIS pH 5.5, 27% PEG 3350 and 2 mM acetyl-CoA