6RF3
Crystal structure of the potassium-pumping G263F mutant of the light-driven sodium pump KR2 in the pentameric form, pH 8.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.003 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 131.680, 239.685, 134.584 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.970 - 2.400 |
| R-factor | 0.1741 |
| Rwork | 0.172 |
| R-free | 0.20460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6REW |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.704 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.6.3) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.970 | 47.970 | 2.450 |
| High resolution limit [Å] | 2.400 | 12.470 | 2.400 |
| Rmerge | 0.131 | 0.028 | 2.283 |
| Rmeas | 0.142 | 0.031 | 2.467 |
| Rpim | 0.055 | 0.012 | 0.928 |
| Total number of observations | 558350 | 4297 | 31500 |
| Number of reflections | 83371 | 665 | 4517 |
| <I/σ(I)> | 10.3 | 34.2 | 0.9 |
| Completeness [%] | 100.0 | 98.2 | 100 |
| Redundancy | 6.7 | 6.5 | 7 |
| CC(1/2) | 0.998 | 0.999 | 0.425 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 3.4 M Sodium Malonate pH 8.0 |
