6RBF
Mucin 2 D3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-24 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 152.461, 156.925, 93.584 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.350 - 2.704 |
| R-factor | 0.2199 |
| Rwork | 0.217 |
| R-free | 0.28330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6n29 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.323 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.350 | 2.801 |
| High resolution limit [Å] | 2.704 | 2.704 |
| Rpim | 0.087 | 0.606 |
| Number of reflections | 62040 | 6087 |
| <I/σ(I)> | 9.92 | |
| Completeness [%] | 99.8 | 98.93 |
| Redundancy | 13.5 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 105 mM lithium sulfate, 100 mM citrate/phosphate buffer, pH 4.2, 10% glycerol, and 14% PEG 1500 |
