6RA8
Horse spleen apoferritin under 2 kBar of argon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.771 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 180.873, 180.873, 180.873 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.260 - 2.000 |
| R-factor | 0.1736 |
| Rwork | 0.171 |
| R-free | 0.22111 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2w0o.pdb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.332 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | FFT |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.260 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.102 | 0.679 |
| Rpim | 0.012 | 0.086 |
| Number of reflections | 17689 | 2516 |
| <I/σ(I)> | 40.1 | 8 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 67.6 | 61.9 |
| CC(1/2) | 1.000 | 0.978 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | Ammonium sulfate 0.6 M + cadmium sulfate 0.1 M |
