6R9P

Structure of Saccharomyces cerevisiae apo Pan2 pseudoubiquitin hydrolase-RNA exonuclease (UCH-Exo) module in complex with AAUUAA RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	I 2 2 2
Unit cell lengths	91.885, 117.091, 255.888
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	86.478 - 2.980
R-factor	0.2635
Rwork	0.261
R-free	0.30860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4czw
Data reduction software	XDS
Data scaling software	xia2 (1.12)
Phasing software	PHASER (2.8)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	106.450	3.060
High resolution limit [Å]	2.980	2.980
Rmerge	0.065	1.512
Number of reflections	28662	4551
<I/σ(I)>	13.5	1.1
Completeness [%]	100.0	100
Redundancy	6.6	6.21
CC(1/2)	0.999	0.726

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.4 M ammonium phosphate