6R9I

Structure of Saccharomyces cerevisiae apo Pan2 pseudoubiquitin hydrolase-RNA exonuclease (UCH-Exo) module

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	I 2 2 2
Unit cell lengths	91.373, 117.075, 255.319
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	86.030 - 3.000
R-factor	0.2456
Rwork	0.243
R-free	0.28950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4czw
Data reduction software	XDS
Data scaling software	xia2 (1.12)
Phasing software	PHASER (2.8)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	106.410	3.080
High resolution limit [Å]	3.000	3.000
Rmerge	0.093	1.761
Number of reflections	27902	1350
<I/σ(I)>	12.4	1.3
Completeness [%]	100.0	99.9
Redundancy	6.6	6.6
CC(1/2)	0.998	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.4 M ammonium phosphate