6R82
Crystal structure of the TLDc domain of Skywalker/TBC1D24 from Drosophila melanogaster
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-21 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9789 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 89.483, 89.483, 135.069 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.369 - 2.046 |
R-factor | 0.1963 |
Rwork | 0.195 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4acj |
RMSD bond length | 0.008 |
RMSD bond angle | 0.867 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.380 | 2.170 |
High resolution limit [Å] | 2.046 | 2.046 |
Rmerge | 0.094 | 0.972 |
Number of reflections | 35238 | 5519 |
<I/σ(I)> | 19.2 | |
Completeness [%] | 99.8 | 99.2 |
Redundancy | 15.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 28% PEG 3350, 0.1 M Hepes pH 7.5 and 0.6M ammonium citrate |