6R80

Structure of AFF4 C-terminal homology domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97925
Spacegroup name	I 21 21 21
Unit cell lengths	41.530, 79.480, 185.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.360 - 2.200
R-factor	0.2334
Rwork	0.232
R-free	0.25150
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.460
Data reduction software	XDS (VERSION Nov 11, 2017)
Data scaling software	XSCALE (VERSION Jan 26, 2018)
Phasing software	HKL2Map (Version 0.4.e-beta)
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.260
High resolution limit [Å]	2.200	2.200
Number of reflections	16107	2204
<I/σ(I)>	11.04	1.11
Completeness [%]	100.0	100
Redundancy	6.97	6.85
CC(1/2)	0.999	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291.15	PEG 8000 14%-16% KH2PO4 0.04 M Glycerol 20%