6R6M
Kusta0087/Kusta0088 Complex purified from Kuenenia stuttgartiensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 44.300, 130.360, 45.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.370 - 1.700 |
| R-factor | 0.1895 |
| Rwork | 0.188 |
| R-free | 0.21030 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.972 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmeas | 0.060 | 0.610 |
| Number of reflections | 29424 | 4287 |
| <I/σ(I)> | 17.6 | 2.2 |
| Completeness [%] | 98.8 | 93.4 |
| Redundancy | 6.3 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 20-25% PEG3000, 0.1 M Na-citrate |
