6R6M

Kusta0087/Kusta0088 Complex purified from Kuenenia stuttgartiensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	44.300, 130.360, 45.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.370 - 1.700
R-factor	0.1895
Rwork	0.188
R-free	0.21030
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	0.972
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.000	1.800
High resolution limit [Å]	1.700	1.700
Rmeas	0.060	0.610
Number of reflections	29424	4287
<I/σ(I)>	17.6	2.2
Completeness [%]	98.8	93.4
Redundancy	6.3	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	20-25% PEG3000, 0.1 M Na-citrate