6R65

Crystal Structure of human TMEM16K / Anoctamin 10 (Form 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	113.158, 152.267, 153.703
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.690 - 3.500
R-factor	0.238
Rwork	0.237
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5oc9
RMSD bond length	0.008
RMSD bond angle	0.900
Data reduction software	XDS (VERSION Oct 15, 2015)
Data scaling software	XDS (VERSION Oct 15, 2015)
Phasing software	PHASER (2.6.1)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.196	3.606
High resolution limit [Å]	3.497	3.500
Rmerge	0.130	0.703
Rmeas	0.178	0.960
Rpim	0.120	0.651
Number of reflections	26237	1312
<I/σ(I)>	7.1	1.7
Completeness [%]	76.4	44.1
Redundancy	3.6	3.5
CC(1/2)	0.998	0.508

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1M calcium acetate 0.1M HEPES pH 7.0 22% PEG400 1CMC C12E9