6R5W
Crystal structure of the receptor binding protein (gp15) of Listeria phage PSA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 134.700, 134.700, 79.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.090 - 1.700 |
| R-factor | 0.1917 |
| Rwork | 0.191 |
| R-free | 0.21080 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.231 |
| Data scaling software | XDS |
| Phasing software | SHELXDE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.090 | 1.761 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.071 | 2.323 |
| Rmeas | 0.075 | 2.447 |
| Rpim | 0.023 | 0.763 |
| Number of reflections | 91053 | 8999 |
| <I/σ(I)> | 17.76 | 1.01 |
| Completeness [%] | 100.0 | 99.98 |
| Redundancy | 10.2 | |
| CC(1/2) | 0.999 | 0.386 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 292 | 0.1 M Na Acetate, pH 4.6, 30% (v/v) PEG 400, and 0.1 M Cadmium Chloride |
