6R5R
The crystal structure of Glycoside Hydrolase BglX inactive mutant D286N from P. aeruginosa in complex with cellobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979257 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.002, 73.580, 81.362 |
| Unit cell angles | 65.60, 73.67, 69.70 |
Refinement procedure
| Resolution | 45.370 - 1.650 |
| R-factor | 0.13608 |
| Rwork | 0.135 |
| R-free | 0.16084 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tf0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.826 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.370 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rpim | 0.060 | 0.390 |
| Number of reflections | 160758 | 7738 |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 96.3 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM Bis Tris, pH 5.5, 17% PEG 10000 and 100 mM ammonium acetate |
