6R4S

Crystal structure of the Pri1 subunit of human primase bound to ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97898
Spacegroup name	C 2 2 21
Unit cell lengths	110.130, 117.270, 151.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.320 - 2.750
R-factor	0.2296
Rwork	0.228
R-free	0.26860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rb4
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260_000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.320	2.910
High resolution limit [Å]	2.750	2.750
Rmeas	0.177	0.917
Number of reflections	25616	3730
<I/σ(I)>	5.46	1.04
Completeness [%]	96.8
Redundancy	4.95
CC(1/2)	0.984	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	23% PEG3350, 10% ethylene glycol, 200 mM Na/K tartrate