6R4Q
Crystal structure of the periplasmic nickel-binding protein NikA from Escherichia coli in complex with Ru(bpza)CO H2O Cl
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2015-02-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979758 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.629, 94.101, 124.418 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.520 - 1.900 |
| R-factor | 0.18036 |
| Rwork | 0.178 |
| R-free | 0.23191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zlq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.560 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.520 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 564360 | 11369 |
| <I/σ(I)> | 16.06 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1M Na acetate pH 4.6 1.8 M ammonium sulfate |
