6R1M
Crystal structure of E. coli seryl-tRNA synthetase complexed to seryl sulfamoyl adenosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.070, 63.520, 75.890 |
| Unit cell angles | 75.44, 70.25, 89.20 |
Refinement procedure
| Resolution | 59.180 - 1.500 |
| R-factor | 0.163 |
| Rwork | 0.162 |
| R-free | 0.18000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dq3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.290 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 163583 | 7950 |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 95.5 | |
| Redundancy | 2.4 | |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M sodium phosphate monobasic monohydrate, pH 4.7 20 % w/v PEG 3350 25 % ethylene glycol |
