6R1J
Structure of the soluble AhlC triple head mutant of the tripartite alpha-pore forming toxin, AHL, from Aeromonas hydrophila.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 103 |
Detector technology | PIXEL |
Collection date | 2018-07-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97951 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 88.538, 88.538, 290.975 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 76.680 - 1.920 |
R-factor | 0.23658 |
Rwork | 0.234 |
R-free | 0.27796 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6h2e |
RMSD bond length | 0.009 |
RMSD bond angle | 1.613 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.860 | 1.900 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 52765 | 2550 |
<I/σ(I)> | 16.1 | |
Completeness [%] | 100.0 | |
Redundancy | 18.7 | |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289.15 | 0.1M imidazole pH 8.0, 10% PEG 8000 |