6R1I

Structure of porcine Aichi virus polymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	P 61 2 2
Unit cell lengths	170.448, 170.448, 231.339
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.128 - 2.634
R-factor	0.1916
Rwork	0.191
R-free	0.22190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	polyalanine model based on 4nz0
RMSD bond length	0.004
RMSD bond angle	0.764
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.130	2.730
High resolution limit [Å]	2.634	2.634
Number of reflections	59076	5790
<I/σ(I)>	33.96
Completeness [%]	100.0
Redundancy	21.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1.7M (NH4)2SO4, 1.7% (v/v) PEG400, 15% (v/v) glycerol, 85 mM HEPES pH 7.5