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6R1C

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with compound 12a

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X10SA
Synchrotron siteSLS
BeamlineX10SA
Temperature [K]100
Detector technologyPIXEL
Collection date2018-02-14
DetectorDECTRIS PILATUS 6M-F
Wavelength(s)1
Spacegroup nameP 21 21 21
Unit cell lengths56.656, 59.020, 88.593
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution49.120 - 1.500
R-factor0.19463
Rwork0.194
R-free0.21537
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4v2y
RMSD bond length0.012
RMSD bond angle1.810
Data reduction softwareXDS
Data scaling softwareXDS
Refinement softwareREFMAC (5.8.0189)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]49.1201.590
High resolution limit [Å]1.5001.500
Rmerge0.1100.790
Number of reflections485557572
<I/σ(I)>14.292.32
Completeness [%]99.597.2
Redundancy12.612.6
CC(1/2)1.0000.870
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP2940.4 M Ammonium phosphate

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