6R18
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with compound 11a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.630, 59.592, 88.894 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.450 - 1.350 |
| R-factor | 0.17409 |
| Rwork | 0.173 |
| R-free | 0.19732 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v2y |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.148 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.450 | 1.430 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.074 | 0.960 |
| Number of reflections | 66849 | 10587 |
| <I/σ(I)> | 17.62 | 1.97 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 12.7 | 12.4 |
| CC(1/2) | 0.999 | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.4 M Ammonium phosphate |
